The Importance of PFGE and Rapd Methods in Determination of Philogenetic Relation among E. Coli Sustains Isolated from Extraintestinal Infection and whose Virulance Factors are Defined

In this study, 155 Escherichia coli strains were isolated to investigatethe association between PFGE and RAPD. The freeze-thaw method was applied for RAPD. The results showed PGFE had a higher discrimination power than RAPD-PCR. With the both methodsjust13(30.9%) samples showed clonal relation, the relation might be a random mutation rather than epidemic since the strains were isolated from the in-patient ofdifferent clinics.The study concluded that RAPD method is more advantageous than PEGE method in terms of the phylotyping and interpretation of obtained results.

Keywords: E.coli , Freeze-thaw, Random mutation, PFGE method, Phylotyping